UW Department of Ophthalmology: IZABELA SOKAL, Ph.D

• Sokal I , Alekseev A, and Palczewski K: Photoreceptor Guanylate Cyclase variants: cGMP production under control. Acta Biochim Pol. 2003; 50(4): 1075-95.

• Sokal I , Hu G, Liang Y, Mao M, Wensel TG, and Palczewski K: Identification of protein kinase C isozymes responsible for the phosphorylation of photoreceptor-specific RGS9-1 at SER475 . J BiolChem 2003; 278: 8316-25.

• Gorczyca WG, Sokal I : GCAPs: Ca 2+ -Sensitive regulators of RetGC. Adv Exp Med Biol 2002; 514:319-32.

• Rex T, Farris RN, Lewis GP, Linberg KA, Sokal I , Fisher SK: A Survey of Molecular Expression by Photoreceptors after Experimental Retinal Detachment. Invest Ophthalmol Vis Sci. 2002; 43:1234-1247.

• Howe KA, Pennesi ME, Sokal I et al. GCAP1 rescues rod photoreceptor response in GCAP1/GCAP2 knockout mice . EMBO J 2002; 21:1545-1554 .

• Haeseleer F, Imanishi Y, Sokal I , Filipek S, Palczewski K: Calcium binding proteins (CaBPs): intracellular sensors from the calmodulin superfamily. Biochem Biophys Res Comm 2002; 290:615-623.

• ImanishiY, Li N, Sokal I , Sowa ME, Lichtarge O, Wensel TG, Saperstien DA, Baehr W, Palczewski K: Characterization of retinal guanylyl-cyclase activating protein 3(GCAP3) from zebrafish to man. Eur. J. Neuroscience 2002; 15:63-78.

• Sokal I , Alekseev A, Baehr W, Haeseleer F, Palczewski K : Soluble fusion proteins between single transmembrane photoreceptor guanylyl cyclases and their activators. Biochemistry 2002; 41:251-7.

• Sokal I , Pulvermüller A, Buczylko J, Hofmann K-P, Palczewski K : Rhodopsin and its kinase. Meth Enzymol 2002; 343:578-600.

• Sokal I , Li N, Klug CS, Filipek S, Hubbell WL, Baehr W, Palczewski K : Calcium-sensitive regions of GCAP1 as observed by chemical modificatons, fluorescence, and EPR spectroscopies. J Biol Chem 2001; 27.

• Li N, Sokal I , Bronson JD, Palczewski K , Baehr W: Identification of functional regions of guanylate cyclase-activating protein 1 (GCAP1) using GCAP1/GCIP chimeras. Biol Chem 2001; 382: 1179-88.

• Mendez A, Burns ME, Sokal I , Dizhoor AM, Baehr W, Palczewski K , Baylor DA, Chen J: Role of guanylate cyclase-activating proteins (GCAPs) in setting the flash sensitivity of rod photoreceptors. Proc Natl Acad Sci USA 2001; 98:9948-53.

• Sokal I , Li N, Verlinde CL, Haeseleer F, Baehr W, Palczewski K. Ca(2+)-binding proteins in the retina: from discovery to etiology of human disease(1). Biochim Biophys Acta. 2000 Dec 20; 1498(2-3): 233-51.

• McBee JK, Kuksa V, Alvarez R, de Lera AR, Prezhdo O, Haeseleer F, Sokal I , Palczewski K . Isomerization of all-trans-retinol to cis-retinols in bovine retinal pigment epithelial cells: dependence on the specificity of retinoid-binding proteins. Biochemistry. 2000 Sep 19; 39(37): 11370-80

• Haeseleer F, Sokal I , Verlinde CL, Erdjument-Bromage H, Tempst P, Pronin AN, Benovic JL, Fariss RN, Palczewski K. Five members of a novel Ca(2+)-binding protein (CABP) subfamily with similarity to calmodulin. J Biol Chem. 2000 Jan 14; 275(2): 1247-60.

• Sokal I , Otto-Bruc AE, Surgucheva I, Verlinde CL, Wang CK, Baehr W, Palczewski K. Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration. J Biol Chem. 1999 Jul 9; 274(28): 19829-37.

• Haeseleer F, Sokal I , Li N, Pettenati M, Rao N, Bronson D, Wechter R, Baehr W, Palczewski K. Molecular characterization of a third member of the guanylyl cyclase-activating protein subfamily. J Biol Chem. 1999 Mar 5;274(10):6526-35.

• Sokal I , Haeseleer F, Arendt A, Adman ET, Hargrave PA, Palczewski K. Identification of a guanylyl cyclase-activating protein-binding site within the catalytic domain of retinal guanylyl cyclase 1. Biochemistry. 1999 Feb 2; 38(5): 1387-93.

• Sokal I , Janusz M, Lisowski J. Interaction of IgG immunoglobulins with the guinea pig peritoneal macrophage Fc gamma receptors. Effect on the association of the receptors with the membrane skeleton and the cytoskeleton. Int J Biochem Cell Biol. 1998 Dec; 30(12): 1353-65.

• Sokal I , Janusz M, Miecznikowska H, Kupryszewski G, Lisowski J. Effect of colostrinin, an immunomodulatory proline-rich polypeptide from ovine colostrum, on sialidase and beta-galactosidase activities in murine thymocytes. Arch Immunol Ther Exp (Warsz). 1998; 46(3): 193-8.

• Sokal I , Li N, Surgucheva I, Warren MJ, Payne AM, Bhattacharya SS, Baehr W, Palczewski K. GCAP1 (Y99C) mutant is constitutively active in autosomal dominant cone dystrophy. Mol Cell. 1998 Jul; 2(1): 129-33.

• Cowan CW, Fariss RN, Sokal I , Palczewski K, Wensel TG.High expression levels in cones of RGS9, the predominant GTPase accelerating protein of rods. Proc Natl Acad Sci U S A. 1998 Apr 28;95 (9):5351-6.

• Rudnicka-Nawrot M, Surgucheva I, Hulmes JD, Haeseleer F, Sokal I , Crabb JW, Baehr W, Palczewski K. Changes in biological activity and folding of guanylate cyclase-activating protein 1 as a function of calcium. Biochemistry. 1998 Jan 6; 37(1): 248-57.

• Sokal I , Kulacz A, Gorczyca W, Janusz M, Lisowski J. Guinea pig peritoneal macrophages. Differential effects of lectins on interaction with IgG immunoglobulins. Cell Biochem Funct. 1995 Mar; 13(1): 25-30

• Sokal I . Fc gamma receptors. Properties and participation in IgG binding and other biological processes. Postepy Hig Med Dosw. 1995; 49(1): 47-51. Review. Polish.


	Education (including Professional Experience)
	1992	M.Sc. (Pharmacology), Academy of Medicine, Faculty of Pharmacy, Wroclaw, Poland
	1992 – 1995	Research Assistant, Laboratory of Immunochemistry, Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
	1995 – 1997	Senior Assistant, Laboratory of Immunochemistry, Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
	1996	Ph.D. (Biology) Institute of Immunology and Experimental Therapy, Wroclaw, Poland
	Postgraduate Training
	1997-2003	Senior Fellow, Department of Ophthalmology, University of Washington, Seattle. Advisor: Krzysztof Palczewski, Ph.D.
	Faculty Positions Held:
	2003-date	Research Assistant Professor, Department of Ophthalmology, University of Washington, Seatttle.
	Honors
	2000	Awarded First Prize for “Best Abstract in Basic Science by a Resident or Fellow,” Department of Ophthalmology, University of Washington.
	1997	Best Doctorate Defense Award by Institute of Immunology, Polish Academy of Science, Wroclaw
	Bibliography
	*Published and In Press:*
	• Sokal I , Alekseev A, and Palczewski K: Photoreceptor Guanylate Cyclase variants: cGMP production under control. Acta Biochim Pol. 2003; 50(4): 1075-95. • Sokal I , Hu G, Liang Y, Mao M, Wensel TG, and Palczewski K: Identification of protein kinase C isozymes responsible for the phosphorylation of photoreceptor-specific RGS9-1 at SER475 . J BiolChem 2003; 278: 8316-25. • Gorczyca WG, Sokal I : GCAPs: Ca 2+ -Sensitive regulators of RetGC. Adv Exp Med Biol 2002; 514:319-32. • Rex T, Farris RN, Lewis GP, Linberg KA, Sokal I , Fisher SK: A Survey of Molecular Expression by Photoreceptors after Experimental Retinal Detachment. Invest Ophthalmol Vis Sci. 2002; 43:1234-1247. • Howe KA, Pennesi ME, Sokal I et al. GCAP1 rescues rod photoreceptor response in GCAP1/GCAP2 knockout mice . EMBO J 2002; 21:1545-1554 . • Haeseleer F, Imanishi Y, Sokal I , Filipek S, Palczewski K: Calcium binding proteins (CaBPs): intracellular sensors from the calmodulin superfamily. Biochem Biophys Res Comm 2002; 290:615-623. • ImanishiY, Li N, Sokal I , Sowa ME, Lichtarge O, Wensel TG, Saperstien DA, Baehr W, Palczewski K: Characterization of retinal guanylyl-cyclase activating protein 3(GCAP3) from zebrafish to man. Eur. J. Neuroscience 2002; 15:63-78. • Sokal I , Alekseev A, Baehr W, Haeseleer F, Palczewski K : Soluble fusion proteins between single transmembrane photoreceptor guanylyl cyclases and their activators. Biochemistry 2002; 41:251-7. • Sokal I , Pulvermüller A, Buczylko J, Hofmann K-P, Palczewski K : Rhodopsin and its kinase. Meth Enzymol 2002; 343:578-600. • Sokal I , Li N, Klug CS, Filipek S, Hubbell WL, Baehr W, Palczewski K : Calcium-sensitive regions of GCAP1 as observed by chemical modificatons, fluorescence, and EPR spectroscopies. J Biol Chem 2001; 27. • Li N, Sokal I , Bronson JD, Palczewski K , Baehr W: Identification of functional regions of guanylate cyclase-activating protein 1 (GCAP1) using GCAP1/GCIP chimeras. Biol Chem 2001; 382: 1179-88. • Mendez A, Burns ME, Sokal I , Dizhoor AM, Baehr W, Palczewski K , Baylor DA, Chen J: Role of guanylate cyclase-activating proteins (GCAPs) in setting the flash sensitivity of rod photoreceptors. Proc Natl Acad Sci USA 2001; 98:9948-53. • Sokal I , Li N, Verlinde CL, Haeseleer F, Baehr W, Palczewski K. Ca(2+)-binding proteins in the retina: from discovery to etiology of human disease(1). Biochim Biophys Acta. 2000 Dec 20; 1498(2-3): 233-51. • McBee JK, Kuksa V, Alvarez R, de Lera AR, Prezhdo O, Haeseleer F, Sokal I , Palczewski K . Isomerization of all-trans-retinol to cis-retinols in bovine retinal pigment epithelial cells: dependence on the specificity of retinoid-binding proteins. Biochemistry. 2000 Sep 19; 39(37): 11370-80 • Haeseleer F, Sokal I , Verlinde CL, Erdjument-Bromage H, Tempst P, Pronin AN, Benovic JL, Fariss RN, Palczewski K. Five members of a novel Ca(2+)-binding protein (CABP) subfamily with similarity to calmodulin. J Biol Chem. 2000 Jan 14; 275(2): 1247-60. • Sokal I , Otto-Bruc AE, Surgucheva I, Verlinde CL, Wang CK, Baehr W, Palczewski K. Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration. J Biol Chem. 1999 Jul 9; 274(28): 19829-37. • Haeseleer F, Sokal I , Li N, Pettenati M, Rao N, Bronson D, Wechter R, Baehr W, Palczewski K. Molecular characterization of a third member of the guanylyl cyclase-activating protein subfamily. J Biol Chem. 1999 Mar 5;274(10):6526-35. • Sokal I , Haeseleer F, Arendt A, Adman ET, Hargrave PA, Palczewski K. Identification of a guanylyl cyclase-activating protein-binding site within the catalytic domain of retinal guanylyl cyclase 1. Biochemistry. 1999 Feb 2; 38(5): 1387-93. • Sokal I , Janusz M, Lisowski J. Interaction of IgG immunoglobulins with the guinea pig peritoneal macrophage Fc gamma receptors. Effect on the association of the receptors with the membrane skeleton and the cytoskeleton. Int J Biochem Cell Biol. 1998 Dec; 30(12): 1353-65. • Sokal I , Janusz M, Miecznikowska H, Kupryszewski G, Lisowski J. Effect of colostrinin, an immunomodulatory proline-rich polypeptide from ovine colostrum, on sialidase and beta-galactosidase activities in murine thymocytes. Arch Immunol Ther Exp (Warsz). 1998; 46(3): 193-8. • Sokal I , Li N, Surgucheva I, Warren MJ, Payne AM, Bhattacharya SS, Baehr W, Palczewski K. GCAP1 (Y99C) mutant is constitutively active in autosomal dominant cone dystrophy. Mol Cell. 1998 Jul; 2(1): 129-33. • Cowan CW, Fariss RN, Sokal I , Palczewski K, Wensel TG.High expression levels in cones of RGS9, the predominant GTPase accelerating protein of rods. Proc Natl Acad Sci U S A. 1998 Apr 28;95 (9):5351-6. • Rudnicka-Nawrot M, Surgucheva I, Hulmes JD, Haeseleer F, Sokal I , Crabb JW, Baehr W, Palczewski K. Changes in biological activity and folding of guanylate cyclase-activating protein 1 as a function of calcium. Biochemistry. 1998 Jan 6; 37(1): 248-57. • Sokal I , Kulacz A, Gorczyca W, Janusz M, Lisowski J. Guinea pig peritoneal macrophages. Differential effects of lectins on interaction with IgG immunoglobulins. Cell Biochem Funct. 1995 Mar; 13(1): 25-30 • Sokal I . Fc gamma receptors. Properties and participation in IgG binding and other biological processes. Postepy Hig Med Dosw. 1995; 49(1): 47-51. Review. Polish.